Cotton microtubule-associated protein GhMAP20L5 mediates fiber elongation through the interaction with the tubulin GhTUB13. (February 2023)

• Record Type: Journal Article
• Authors: Song, Qingwei; Gao, Wanting; Du, Chuanhui; Wang, Jin; Zuo, Kaijing
• Is Part Of: Plant science. Volume 327(2023)
• Publication Date: 2023-02
• Subjects: Cotton -- Fiber elongation -- GhMAP20L5 -- Microtubules (MTs) -- Tubulin; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2022.111545
• Languages: English
• ISSNs: 0168-9452

Abstract

Abstract: Targeting proteins for Xklp2 (TPX2s) comprise a class of MAPs that are essential for plant growth and development by regulating the dynamic changes of microtubules (MTs) and proper formation of cytoskeleton. However, the function of TPX2 proteins in cotton fiber development remains poorly understood. Here, we identified the function of a fiber elongation-specific TPX2 protein, GhMAP20L5, in cotton. Suppressed GhMAP20L5 gene expression in cotton ( GhMAP20L5i ) significantly reduced fiber elongation rate, fiber length and lint percentage. GhMAP20L5i fibers had thinner and looser secondary cell walls (SCW), and incompact helix twists. GhMAP20L5 specifically interacted with the tubulin GhTUB13 on the cytoskeleton. Gene coexpression analysis showed that GhMAP20L5 involved in multiple pathways related to cytoskeleton establishment and fiber cell wall formation and affected cellulase genes expressions. In summary, our results revealed that GhMAP20L5 is important for fiber development by regulating cytoskeleton establishment and the cellulose deposition in cotton. Highlights: GhMAP20L5 is a microtubule-associated protein (MAP) differentially expressed in cotton fiber development. Silenced GhMAP20L5 in cotton reduced fiber elongation rate, fiber length and lint percentage. Inhibition of GhMAP20L5 affected the secondary cell wall architecture of cotton fibers. GhMAP20L5 involved in fiber elongation through the interaction with GhTUB13.