Expression profile and molecular function of beclin-1 in Epinephelus akaara in response to immune stimuli and oxidative stress. Issue 132 (January 2023)

• Record Type: Journal Article
• Authors: Nadarajapillai, Kishanthini; Udayantha, H.M.V.; Sellaththurai, Sarithaa; Kasthuriarachchi, T.D.W.; Kim, Gaeun; Lee, Jehee
• Is Part Of: Fish & shellfish immunology. Issue 132(2023)
• Publication Date: 2023-01
• Subjects: Beclin-1 -- Ephinephelus akaara -- Immune response -- VHSV -- Oxidative stress; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.108490
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Beclin-1, the mammalian ortholog of the yeast autophagy-related gene 6 (Atg 6), is a key regulator of autophagy. A variety of health and disease conditions in mammals are intricately related to the broad spectrum of beclin-1 functions. Nevertheless, few studies have investigated the role of beclin-1 in fish. In this study, we identified and cloned the beclin-1 cDNA ( EaBECN-1 ) of Epinephelus akaara (red-spotted grouper) and carried out in silico analysis, tissue-specific expression analysis, immune challenge experiment, and in vitro analysis of its roles against viral infection and oxidative stress. The open reading frame was 1344 bp long and encoded 447 amino acids with a molecular weight of 51.2 kDa. Beclin-1 consisted of a conserved N-terminal BH3 and APG6 domains, and shared more than 88% identity with other vertebrates, according to a pairwise sequence alignment. EaBECN-1 expression profile analysis in E. akaara revealed that it is mostly expressed in the blood. Moreover, transcriptional modulation of EaBECN-1 was observed following stimulation with lipopolysaccharide (LPS), polyinosinic-polycytidylic acid (poly (I:C)), and nervous necrosis virus. During the viral hemorrhagic septicemia virus challenge, increased viral gene expression was observed at 12 h post-infection in FHM cells ectopically expressing EaBECN-1, and decreased thereafter at 24 h post-infection compared to control cells. However, increased antiviral gene expression at 12 and 24 h confirmed the antiviral function of EaBECN-1. Furthermore, EaBECN-1 overexpression protected the cells against H2 O2 -mediated apoptosis, as evidenced by the MTT assay, analysis of mRNA expression levels of apoptotic genes, and AO-EtBr staining. Overall, our study demonstrated the protective role of EaBECN-1 against viral pathogenesis and oxidative stress through autophagy, increasing our understanding of the role of beclin-1 in fish. Highlights: Beclin-1 was identified and characterized in red-spotted grouper ( EaBECN-1 ). EaBECN-1 was ubiquitously expressed in all examined tissues. The significant transcript modulation of EaBECN-1 was observed during the immune challenges. The overexpressed EaBECN-1 showed antiviral activity against VHSV and an anti-apoptotic effect on oxidative stress.