Bufotalin ameliorates ovalbumin-induced allergic rhinitis by restoring the Tregs. (January 2023)

• Record Type: Journal Article
• Authors: Ji, Qiang; Ren, Shoulei; Li, Ling
• Is Part Of: Microbial pathogenesis. Volume 174(2023)
• Publication Date: 2023-01
• Subjects: Bufotalin -- Allergic rhinitis -- Tregs -- Inflammation; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2022.105918
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Background: Allergic rhinitis (AR) is one of the most common inflammatory diseases. IgE, inflammatory cytokine production and Th17/Tregs imbalance have been implicated in AR pathogenesis. Bufotalin, a component extracted from toad venom skin secretions and auricular glands, has anti-inflammatory activity and regulates Th17/Tregs balance. Here, the effects of bufotalin on AR were explored. Methods: The AR mice model was established using ovalbumin (OVA). AR mice were treated with bufotalin started on Day 22 with various doses (1, 10, 100 μg or 1 mg per mouse) every day to Day 30. The sneezing and rubbing frequencies were counted. Serum levels of IL-1β, IL-10 and OVA-specific IgE were measured. The superficial cervical lymph nodes were harvested and the percentage of Tregs in lymph node was determined using CD4 and Foxp3 markers. Results: OVA treatment successfully induced AR model in mice with significantly increased sneezing and rubbing frequency, elevated levels of serum histamine, IL-1β, IL-10 and OVA-specific IgE. Bufotalin treatment significantly ameliorated AR symptoms, with reduced histamine, IgE and IL-1β levels, as well as sneezing and rubbing frequency. Moreover, bufotalin treatment decreased the serum levels of IL-1β, IL-10 and OVA-specific IgE in AR mice. Conlcusion: Bufotalin ameliorated allergic rhinitis symptoms in AR mice by restoring Tregs in lymph node. Highlights: Bufotalin ameliorates AR symptoms. Bufotalin attenuates the production of inflammatory cytokines. Bufotalin restores Tregs in lymph node.