Bactericidal effects of plasma‐activated saline prepared by surface dielectric barrier discharge with different dielectric layers and working gases. Issue 1 (5th September 2022)

• Record Type: Journal Article
• Authors: Huang, Lingling; Guo, Li; Zhao, Pengyu; Jing, Xixi; Zhang, Fugao; Niyazi, Gulimire; Li, Tianhui; Qi, Yu; Yan, Jinwei; Jia, Yikang; Liu, Dingxin
• Is Part Of: Plasma processes and polymers. Volume 20:Issue 1(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-09-05
• Subjects: bactericidal effect -- crosslinking -- plasma‐activated saline -- reactive species -- surface discharges; Plasma polymerization -- Periodicals; Plasma-enhanced chemical vapor deposition -- Periodicals; Plasma chemistry -- Periodicals
• DOI: 10.1002/ppap.202200110
• Languages: English
• ISSNs: 1612-8850

Abstract

Abstract: The polytetrafluoroethylene (PTFE) serves as a dielectric layer for surface dielectric barrier discharge (SDBD) and the prepared plasma‐activated saline (PAS) exhibited strong bactericidal effects, however, the fluorides are inevitably generated and bring about side effects in biological application. In this study, the SDBD devices with the dielectric layers of quartz or ceramic compared with that with PTFE were utilized to explore the effective and fluoride‐free PAS using different working gases. The Quartz‐PAS prepared with air exhibited a relatively strong bactericidal effect without inducing the DNA‐protein crosslinks (DPCs) in Escherichia coli cells, while the Ceramic‐PAS exhibited a weak bactericidal effect and induced the DPCs formation. This study supplies guidance in the preparation and regulation of PAS for biomedical applications. Abstract : This study aims to precisely compare the bactericidal efficiency of plasma‐activated saline (PAS) prepared by the surface dielectric barrier discharge devices with polytetrafluoroethylene (PTFE), quartz, or ceramic with different working gases. The PTFE‐PAS exhibited the strongest bactericidal effect while the toxic fluorides were inevitably released. The Quartz‐PAS exhibited a relatively strong bactericidal effect without inducing the DNA‐protein crosslinks (DPCs) in bacterial cells, while the Ceramic‐PAS exhibited a weak bactericidal effect and induced DPCs formation.