Erythorbyl laurate suppresses TNF-α-induced adhesion of monocytes to the vascular endothelium. (May 2021)

• Record Type: Journal Article
• Authors: Ha, Su Jeong; Kim, Min Jeong; Park, Joon; Choi, Hyun-Wook; Yu, Hyunjong; Chang, Pahn-Shick; Jung, Sung Keun
• Is Part Of: Journal of functional foods. Volume 80(2021)
• Publication Date: 2021-05
• Subjects: Erythorbyl laurate -- Arteriosclerosis -- Monocyte adhesion -- Vascular inflammation -- VCAM-1 -- NF-κB; EL Erythorbyl laurate -- TNF-α tumor necrosis factor -- VCAM-1 vascular cell adhesion molecule -- HUVEC human umbilical vein endothelial cell -- IKK IκB kinase -- ERK 1/2 extracellular-signal-regulated kinase 1/2 -- JNK c-Jun N-terminal kinases 1/2 -- IL interleukin -- CCL2 monocyte chemoattractant protein-1 -- MAPK mitogen-activated protein kinase -- NF-κB nuclear factor kappa-light-chain-enhancer of activated B cells -- ABTS 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) -- DPPH 1, 1-Diphenyl-2-picrylhydrazyl -- FBS Fetal Bovine Serum -- IRAK1 interleukin-1 receptor associated kinase 1 -- TAK1 transforming growth factor-β-activated kinase 1; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2021.104428
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Oral administration of EL ameliorated TNF-α-induced vascular inflammation. Oral administration of EL inhibited TNF-α-induced monocyte infiltration in the aortic root. Oral administration of EL inhibited TNF-α-induced production of inflammatory cytokines including IL6 and TNFA in mice aorta. EL diminished TNF-α-induced phosphorylation of MAPK and NF-κB signaling molecules. EL suppressed TNF-α-induced TAK1 phosphorylation in HUVECs. Abstract: Erythorbyl laurate (EL) can be produced via lipase-catalyzed esterification between erythorbic acid and lauric acid. In this study, we evaluate the anti-inflammatory effect of EL in the early stage of atherosclerosis. EL suppressed tumor necrosis factor (TNF)-α-induced monocyte adhesion to vascular endothelial cells and expression of vascular cell adhesion molecule (VCAM)-1 in human umbilical vein endothelial cells (HUVECs). Additionally, EL suppressed TNF-α-induced p65/IκB kinase (IKK)/IκB phosphorylation in HUVECs. Western blot analysis of cytosolic and nuclear cell fractions and immunofluorescence showed that EL suppressed TNF-α-induced translocation of p65 from the cytoplasm to the nucleus. EL also inhibited phosphorylation of extracellular-signal-regulated kinase (ERK) 1/2, p38, and c-Jun N-terminal kinases (JNK) 1/2 in HUVECs. EL suppressed TNF-α-induced phosphorylation of Akt, IRAK1, and TAK1 in HUVECs. Quantitative RT-PCR analysis showed that EL significantly suppressed TNF-α-induced interleukin (IL)1B, IL6, TNFA, and CCL2 mRNA expression in HUVECs. Additionally, oral administration of EL suppressed TNF-α-induced IL6 and TNFA expression in the mouse aorta. EL could represent a promising functional nutrient that can be ingested for the prevention of vascular inflammation via decreased monocyte infiltration to the vascular endothelium and suppression of inflammatory nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs) signaling pathways.