Molecular cloning and expression analysis of EIN2, EIN3/EIL, and EBF genes during papaya fruit development and ripening. Issue 2 (3rd July 2021)

• Record Type: Journal Article
• Authors: Zou, Yuan; Ding, Xiaochun; Ye, Lanlan; Zhu, Xiaoyang; Zhang, Lin; Rao, Shen; Chen, Weixin; Li, Xueping
• Is Part Of: New Zealand journal of crop and horticultural science. Volume 49:Issue 2/3(2021)
• Page Start: 151
• Page End: 167
• Publication Date: 2021-07-03
• Subjects: Papaya fruit -- ethylene signal transduction -- fruit development -- fruit ripening -- gene expression; Crops -- New Zealand -- Periodicals; Horticulture -- New Zealand -- Periodicals; Crops -- Periodicals; Horticulture -- Periodicals; Crops; Horticulture; New Zealand; Periodicals; 633.09905
• DOI: 10.1080/01140671.2020.1838571
• Languages: English
• ISSNs: 1175-8783

Abstract

ABSTRACT: In the ethylene signalling pathway, EIN2 is an essential signal transducer linking ethylene perception on endoplasmic reticulum to transcriptional regulation in the nucleus, EIN3/EIL1 transcription factors initiate various responses leading to an ethylene response, and EBFs function in ethylene perception by regulating EIN3/EIL1 turnover. In this work, we isolated and characterised three EIN3 homologs ( CpEIN3a, CpEIN3b, and CpEIL1 ), and two EBF homologs ( CpEBF1 and CpEBF2 ) from papaya fruits. During the fruit development, the transcripts of all these genes were accumulated at a low level before reaching a maximum in the third month, then showed a decrease. Exogenous ethylene application and 1-MCP treatment had different effects on the expression of these genes. The correlations between various physiological characteristics with the gene expressions showed that CpEIN3b, CpEIL1, and CpEBF1/2 were negative regulators in terms of papaya fruit ripening under exogenous ethylene treatment while CpEIN3a was a positive one, CpEIN3a was negatively correlated with the respiration rate and ethylene production of papaya fruits under 1-MCP treatment. It is suggested that CpEIN2, CpEIN3a/b, CpEIL1, and CpEBF1/2 are development- and ripening-related genes in papaya fruits. These results provide new insights into the understanding of the ethylene signalling cascade in papaya fruit development and ripening.