A synthetic biology approach for the transformation of l-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids. Issue 14 (9th January 2015)

• Record Type: Journal Article
• Authors: Gourinchas, Geoffrey; Busto, Eduardo; Killinger, Manuela; Richter, Nina; Wiltschi, Birgit; Kroutil, Wolfgang
• Is Part Of: Chemical communications. Volume 51:Issue 14(2015)
• Page Start: 2828
• Page End: 2831
• Publication Date: 2015-01-09
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c4cc08286a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A synthetic biology approach enabled the design of a single E. coli cell catalyst co-expressing three enzymes (l -AAD, l - or d -HIC and FDH) for the quantitatively transformation of l -amino acids to the corresponding optically pure ( R )- and ( S )-α-hydroxy acids. Abstract : Combinatorial assembly and variation of promoters on a single expression plasmid allowed the balance of the catalytic steps of a three enzyme (l -AAD, HIC, FDH) cascade in E. coli . The designer cell catalyst quantitatively transformed l -amino acids to the corresponding optically pure ( R )- and ( S )-α-hydroxy acids at up to 200 mM substrate concentration.