Enzymatic hydrolysis of chitin pretreated by bacterial fermentation to obtain pure N-acetyl-d-glucosamine. Issue 10 (25th April 2018)

• Record Type: Journal Article
• Authors: Zhang, Alei; Wei, Guoguang; Mo, Xiaofang; Zhou, Ning; Chen, Kequan; Ouyang, Pingkai
• Is Part Of: Green chemistry. Volume 20:Issue 10(2018)
• Page Start: 2320
• Page End: 2327
• Publication Date: 2018-04-25
• Subjects: Environmental chemistry -- Industrial applications -- Periodicals; Environmental management -- Periodicals; 660
• DOI: 10.1039/c8gc00265g
• Languages: English
• ISSNs: 1463-9262

Abstract

Abstract : In this study, we propose a novel biological fermentation method to process chitin powder utilizing the bacteria Chitinolyticbacter meiyuanensis SYBC-H1. Abstract : In this study, we propose a novel biological fermentation method to process chitin powder utilizing the bacteria Chitinolyticbacter meiyuanensis SYBC-H1. The chitin obtained from this process is called CBF, and it was further investigated for N -acetyl-d -glucosamine (GlcNAc) enzymatic production. This fermentative treatment caused chitin powder to acquire a visible fleecy structure, and scanning electron microscopy showed that the surface of the CBF was a fiber-like structure with a diameter ranging from 10 nm to 200 nm. Moreover, Fourier transform infrared spectrometry, X-ray diffraction and gel permeation chromatography revealed that the crystallinity and average molecular weight of the chitin clearly decreased in the absence of deacetylation following bacterial fermentation treatment. The optimal conditions for bacterial treatment of chitin were determined at 25 °C, 200 rpm, and an initial pH of 7.5 for 12 h. Additionally, 19.2 g L −1 of GlcNAc could be obtained with a yield of 96% from 20 g L −1 of CBF in a batch enzymatic reaction within 6 h. This new approach provided an environmentally friendly and mild method for reducing the crystallinity of chitin and enhancing GlcNAc conversion without the use of oxidants, acids, or other environment unfriendly reagents.