A novel two-layer-integrated microfluidic device for high-throughput yeast proteomic dynamics analysis at the single-cell level. Issue 10 (29th September 2020)

• Record Type: Journal Article
• Authors: Chen, Kaiyue; Rong, Nan; Wang, Shujing; Luo, Chunxiong
• Is Part Of: Integrative biology. Volume 12:Issue 10(2020)
• Page Start: 241
• Page End: 249
• Publication Date: 2020-09-29
• Subjects: multilayer integrated -- microfluidics -- cell loading -- budding yeast -- proteomic dynamics; Biology -- Periodicals; Technology -- Periodicals; Biological systems -- Periodicals; 570.5
• DOI: 10.1093/intbio/zyaa018
• Languages: English
• ISSNs: 1757-9694

Abstract

Abstract: Current microfluidic methods for studying multicell strains (e.g., m-types) with multienvironments (e.g., n-types) require large numbers of inlets/outlets (m*n), a complicated procedure or expensive machinery. Here, we developed a novel two-layer-integrated method to combine different PDMS microchannel layers with different functions into one chip by a PDMS through-hole array, which improved the design of a PDMS-based microfluidic system. Using this method, we succeeded in converting 2 × m × n inlets/outlets into m + n inlets/outlets and reduced the time cost of loading processing (from m × n to m) of the device for studying multicell strains (e.g., m-types) in varied multitemporal environments (i.e., n-types). Using this device, the dynamic behavior of the cell-stress-response proteins was studied when the glucose concentration decreased from 2% to a series of lower concentrations. Our device could also be widely used in high-throughput studies of various stress responses, and the new concept of a multilayer-integrated fabrication method could greatly improve the design of PDMS-based microfluidic systems.