Serratia marcescens SCH909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms. Issue 14 (15th July 2021)

• Record Type: Journal Article
• Authors: Gambino, Anahí S; Déraspe, Maxime; Álvarez, Verónica E; Quiroga, María Paula; Corbeil, Jacques; Roy, Paul H; Centrón, Daniela
• Is Part Of: FEMS microbiology letters. Volume 368:Issue 14(2021)
• Publication Date: 2021-07-15
• Subjects: Serratia marcescens -- MARR (multiple antibiotic resistance region); Microbiology -- Periodicals; 579
• DOI: 10.1093/femsle/fnab086
• Languages: English
• ISSNs: 0378-1097

Abstract

ABSTRACT: Serratia marcescens SCH909 is a multidrug resistant strain isolated in 1988 harboring three class 1 integrons. We wondered if these integrons were retained over time and if there were other antimicrobial resistant determinants contributing to its multidrug resistant profile. Genomic analysis showed a fourth multidrug resistance integron, a Tn 7 transposon with dfrA1-sat2-ybeA-ybfA-ybfB-ybgA gene cassettes in the variable region. Insertion sequences were involved in the genesis of novel composite transposons in the L4 subtype plasmid pSCH909, such as Tn 6824 carrying an arsenic regulon and two head to head class 1 integrons surrounded by two complete IS 1 . Remarkably, a novel chromosomal genomic island, SmaR, was identified, closely related to Multiple Antimicrobial Resistance Regions (MARR), usually found in AbaR0-type and AbGRI2-0 from global clones of Acinetobacter baumannii, and in M-type plasmids circulating in Enterobacteriaceae . Maintenance studies showed that the three class 1 integrons were maintained over 1 month without antimicrobial pressure. Since S. marcescens is considered a relevant nosocomial pathogen that can have a wide range of niches – human, plant, animal, soil and inanimate surfaces, our findings support the ability of this species to capture, maintain and spread a broad variety of antimicrobial resistance elements. Abstract : Genomic plasticity of Serratia marcescens capable of hosting multiple antibiotic resistance genes.