The Physcomitrium (Physcomitrella) patens PpKAI2L receptors for strigolactones and related compounds function via MAX2-dependent and -independent pathways. Issue 11 (30th August 2021)

• Record Type: Journal Article
• Authors: Lopez-Obando, Mauricio; Guillory, Ambre; Boyer, François-Didier; Cornu, David; Hoffmann, Beate; Le Bris, Philippe; Pouvreau, Jean-Bernard; Delavault, Philippe; Rameau, Catherine; de Saint Germain, Alexandre; Bonhomme, Sandrine
• Is Part Of: The Plant Cell. Volume 33:Issue 11(2021)
• Page Start: 3487
• Page End: 3512
• Publication Date: 2021-08-30
• DOI: 10.1093/plcell/koab217
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract: In angiosperms, the α/β hydrolase DWARF14 (D14), along with the F-box protein MORE AXILLARY GROWTH2 (MAX2), perceives strigolactones (SL) to regulate developmental processes. The key SL biosynthetic enzyme CAROTENOID CLEAVAGE DIOXYGENASE8 (CCD8) is present in the moss Physcomitrium patens, and PpCCD8-derived compounds regulate moss extension. The PpMAX2 homolog is not involved in the SL response, but 13 PpKAI2LIKE ( PpKAI2L ) genes homologous to the D14 ancestral paralog KARRIKIN INSENSITIVE2 ( KAI2 ) encode candidate SL receptors. In Arabidopsis thaliana, AtKAI2 perceives karrikins and the elusive endogenous KAI2-Ligand (KL). Here, germination assays of the parasitic plant Phelipanche ramosa suggested that PpCCD8-derived compounds are likely noncanonical SLs. (+)-GR24 SL analog is a good mimic for PpCCD8-derived compounds in P. patens, while the effects of its enantiomer (−)-GR24, a KL mimic in angiosperms, are minimal. Interaction and binding assays of seven PpKAI2L proteins pointed to the stereoselectivity toward (−)-GR24 for a single clade of PpKAI2L (eu-KAI2). Enzyme assays highlighted the peculiar behavior of PpKAI2L-H. Phenotypic characterization of Ppkai2l mutants showed that eu-KAI2 genes are not involved in the perception of PpCCD8-derived compounds but act in a PpMAX2-dependent pathway. In contrast, mutations in PpKAI2L-G, and -J genes abolished the response to the (+)-GR24 enantiomer, suggesting that PpKAI2L-G, and -J proteins are receptors for moss SLs. Abstract : The study of moss PpKAI2L receptors for strigolactones and related compounds highlights MORE AXILLARY GROWTH2-dependent and -independent pathways for the perception of these compounds.