A Chromosome—Level Genome Assembly of the Spotted Scat (Scatophagus argus). (30th April 2021)

• Record Type: Journal Article
• Authors: Huang, Yuanqing; Mustapha, Umar Farouk; Huang, Yang; Tian, Changxu; Yang, Wei; Chen, Huapu; Deng, Siping; Zhu, Chunhua; Jiang, Dongneng; Li, Guangli
• Editors: Fraser, Bonnie
• Is Part Of: Genome biology and evolution. Volume 13:Number 6(2021)
• Publication Date: 2021-04-30
• Subjects: spotted scat -- genomics -- PacBio sequencing -- Hi-C proximity mapping -- chromosomal assembly; Genomics -- Periodicals; Genes -- Periodicals; 572.8605
• DOI: 10.1093/gbe/evab092
• Languages: English
• ISSNs: 1759-6653

Abstract

Abstract: The spotted scat, Scatophagus argus is a member of the family Scatophagidae found in Indo-Pacific coastal waters. It is an emerging commercial aquaculture species, particularly in East and Southeast Asia. In this study, the first chromosome-level genome of S. argus was constructed using PacBio and Hi-C sequencing technologies. The genome is 572.42 Mb, with a scaffold N50 of 24.67 Mb. Using Hi-C data, 563.28 Mb (98.67% of the genome) sequences were anchored and oriented in 24 chromosomes, ranging from 12.57 Mb to 30.38 Mb. The assembly is of high integrity, containing 94.26% conserved single-copy orthologues, based on BUSCO analysis. A total of 24, 256 protein-coding genes were predicted in the genome, and 96.30% of the predicted genes were functionally annotated. Evolutionary analysis showed that S. argus diverged from the common ancestor of Japanese puffer ( Takifugu rubripes ) approximately 114.8 Ma. The chromosomes of S. argus showed significant correlation to T. rubripes chromosomes. A comparative genomic analysis identified 49 unique and 90 expanded gene families. These genomic resources provide a solid foundation for functional genomics studies to decipher the economic traits of this species.