Results of an Interlaboratory Comparison of a Liquid Chromatography–Isotope Ratio Mass Spectrometry Method for the Determination of 13C/12C Ratios of Saccharides in Honey. (22nd September 2021)

• Record Type: Journal Article
• Authors: Aries, Eric; De Rudder, Oliver; Kaklamanos, Georgios; Maquet, Alain; Ulberth, Franz
• Is Part Of: Journal of AOAC International. Volume 104:Number 6(2021)
• Page Start: 1698
• Page End: 1702
• Publication Date: 2021-09-22
• Subjects: Agricultural chemistry -- Periodicals; Food -- Analysis -- Periodicals; 543
• DOI: 10.1093/jaoacint/qsab091
• Languages: English
• ISSNs: 1060-3271

Abstract

Abstract: Background: Stable carbon isotope analysis of sugars in honey by LC–isotope ratio mass spectrometry (IRMS) is a useful tool for detecting adulteration of honey with extraneous sugar. Purity criteria based on 13 C/ 12 C ratios of saccharides in honey, determined by LC–IRMS of a large number of authentic honey samples, have been elaborated. However, no interlaboratory comparison (ILC) has yet been performed to estimate the precision of the method under reproducibility conditions. Objective: To address this knowledge gap an ILC involving 14 laboratories and using six honey samples was conducted. Methods: The participants were allowed to use their LC–IRMS-based method of choice for sample preparation and compound separation. Results: The precision figures were estimated according to ISO 5725:1994. The repeatability relative standard deviation (RSDr ) for the determination of δ 1 3 C values of fructose and glucose varied between 0.3 and 0.5%, with 0.3 and 1.0% for disaccharides, and 0.7 and 2.8% for trisaccharides. The RSDR varied between 0.8 and 1.8% for the monosaccharides, 1.0 and 1.5% for disaccharides, and 1.4 and 2.8% for trisaccharides. Conclusion: Based on the obtained precision data the LC–IRMS method for the determination of 13 C/ 12 C ratios of saccharides in honey was considered fit for the conformity assessment of honey with established purity criteria. Highlights: Precision estimates for a LC–IRMS method to determine 13 C/ 12 C ratios of saccharides in honey were obtained through an ILC. The data created can form the basis for the standardization of the method by interested standards-developing organizations for use in official control.