Gold Nanoparticle Enabled Localized Surface Plasmon Resonance on Unique Gold Nanomushroom Structures for On‐Chip CRISPR‐Cas13a Sensing. Issue 1 (27th November 2022)

• Record Type: Journal Article
• Authors: Waitkus, Jacob; Chang, Yu; Liu, Li; Puttaswamy, Srinivasu Valagerahally; Chung, Taerin; Molina Vargas, Adrian M.; Dollery, Stephen J.; O'Connell, Mitchell R.; Cai, Haogang; Tobin, Gregory J.; Bhalla, Nikhil; Du, Ke
• Is Part Of: Advanced materials interfaces. Volume 10:Issue 1(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-11-27
• Subjects: CRISPR‐Cas13a -- finite difference time domain -- gold nanomushroom -- gold nanoparticle -- localized surface plasmon resonance (LSPR) -- microfluidics -- SARS‐CoV‐2; Materials science -- Periodicals; 620.11
• DOI: 10.1002/admi.202201261
• Languages: English
• ISSNs: 2196-7350

Abstract

Abstract: A novel localized surface plasmon resonance (LSPR) system based on the coupling of gold nanomushrooms (AuNMs) and gold nanoparticles (AuNPs) is developed to enable a significant plasmonic resonant shift. The AuNP size, surface chemistry, and concentration are characterized to maximize the LSPR effect. A 31 nm redshift is achieved when the AuNMs are saturated by the AuNPs. This giant redshift also increases the full width of the spectrum and is explained by the 3D finite‐difference time‐domain (FDTD) calculation. In addition, this LSPR substrate is packaged in a microfluidic cell and integrated with a CRISPR‐Cas13a RNA detection assay for the detection of the SARS‐CoV‐2 RNA targets. Once activated by the target, the AuNPs are cleaved from linker probes and randomly deposited on the AuNM substrate, demonstrating a large redshift. The novel LSPR chip using AuNP as an indicator is simple, specific, isothermal, and label‐free; and thus, provides a new opportunity to achieve the next generation multiplexing and sensitive molecular diagnostic system. Abstract : A nanoplasmonic system based on the coupling of gold nanomushrooms and gold nanoparticles is introduced, showing a giant plasmonic resonant shift up to 31 nm. The gold nanoparticles labeled by CRISPR‐Cas13a assay are non‐specifically deposited on the gold nanomushrooms through a microfluidic cell for the accurate recognition of target viral RNA.