Silver nanoparticles for enhancing the efficiency of micropropagation of gray poplar (Populus × canescens Aiton. Sm.). Issue 1 (October 2021)

• Record Type: Journal Article
• Authors: Vasyukova, I; Gusev, A; Zakharova, O; Baranchikov, P; Yevtushenko, N
• Is Part Of: IOP conference series. Volume 875:Issue 1(2021)
• Publication Date: 2021-10
• Subjects: Earth sciences -- Periodicals; Environmental sciences -- Congresses; Environmental sciences -- Periodicals; 550.5
• DOI: 10.1088/1755-1315/875/1/012053
• Languages: English
• ISSNs: 1755-1307

Abstract

Abstract: The main purpose of the work was to determine the prospects for the use of silver nanoparticles in woody plants tissue culture and to identify possible limitations. The paper presents the results of experiments on the use of silver nanoparticles (AgNPs) to increase the efficiency of in vitro tissue culture of woody plant cultures using the example of gray poplar ( Populus × canescens Aiton. Sm .) at various stages of microclonal reproduction. It has been shown that sequential treatment with 7.5 g/L NaOCl for 30 min and 0.3 g/L AgNPs for 5 min allows obtaining 100% phytopathogen-free explants, increasing their viability. Modification of the nutrient medium with AgNPs at concentrations of 1.5…3 μg/L during multiplication and rooting reduces the phytopathogen infestation, and also stimulates the formation of the root system and accelerates the growth of the vegetative part of the shoots. In addition, the combination of photosynthetic and enzymatic activity suggests a rather high potential for stress resistance of experimental microclones in comparison with control ones.