Development and Application of the Sample Support in Transmission Electron Microscopy. Issue 2 (January 2021)

• Record Type: Journal Article
• Authors: Pan, Hengyu
• Is Part Of: IOP conference series. Volume 632:Issue 2(2021)
• Publication Date: 2021-01
• Subjects: Cryo-EM -- Graphene -- Supporting Film -- Amorphous Carbon -- Sample Preparation; Earth sciences -- Periodicals; Environmental sciences -- Congresses; Environmental sciences -- Periodicals; 550.5
• DOI: 10.1088/1755-1315/632/2/022004
• Languages: English
• ISSNs: 1755-1307

Abstract

Abstract: Cryogenic electron microscopy is becoming an increasingly popular technology that allows us to observe the molecules in high-resolution details. With this refined way to study the structures in scientific researches, the observation of the molecules will be expected to make improvements. The conventional methods, such as the X-ray crystallography and the nuclear magnetic resonance spectroscopy, provide the basic observation of ground-state molecules and the measurement of various small molecules. But both of them contain flaws discovered in the continuing development process. Therefore, the application of cryo-EM becomes a broader area to explore. In this review, we will give an overall understanding of the cryogenic electron microscopy. We will discuss the intention behind the invention of the cryo-EM and introduce the main workflow of the experiments. In addition, we will discuss the methods to prepare the sample in a well-condition and compare them among different groups of experiments. More importantly, we will discuss the application of the graphene in cryo-EM and evaluate possible improvements in future development.