The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus. Issue 17 (9th July 2018)

• Record Type: Journal Article
• Authors: Rochat, Tatiana; Bohn, Chantal; Morvan, Claire; Le Lam, Thao Nguyen; Razvi, Fareha; Pain, Adrien; Toffano-Nioche, Claire; Ponien, Prishila; Jacq, Annick; Jacquet, Eric; Fey, Paul D; Gautheret, Daniel; Bouloc, Philippe
• Is Part Of: Nucleic acids research. Volume 46:Issue 17(2018)
• Page Start: 8803
• Page End: 8816
• Publication Date: 2018-07-09
• Subjects: Nucleic acids -- Periodicals; Molecular biology -- Periodicals; 572.805
• DOI: 10.1093/nar/gky584
• Languages: English
• ISSNs: 0305-1048

Abstract

Abstract: RsaE is a regulatory RNA highly conserved amongst Firmicutes that lowers the amount of mRNAs associated with the TCA cycle and folate metabolism. A search for new RsaE targets in Staphylococcus aureus revealed that in addition to previously described substrates, RsaE down-regulates several genes associated with arginine catabolism. In particular, RsaE targets the arginase rocF mRNA via direct interactions involving G-rich motifs. Two duplicated C-rich motifs of RsaE can independently downregulate rocF expression. The faster growth rate of Δ rsaE compared to its parental strain in media containing amino acids as sole carbon source points to an underlying role for RsaE in amino acid catabolism. Collectively, the data support a model in which RsaE acts as a global regulator of functions associated with metabolic adaptation.