Sulfur transformation and bacterial community dynamics in both desulfurization-denitrification biofilm and suspended activated sludge. (January 2022)

• Record Type: Journal Article
• Authors: Li, Wei; Zhen, Yuming; Li, Nan; Wang, Hengqi; Lin, Minghui; Sui, Xiuting; Zhao, Wanying; Guo, Ping; Lin, Jianguo
• Is Part Of: Bioresource technology. Volume 343(2022)
• Publication Date: 2022-01
• Subjects: Biofilm -- Suspended sludge -- Trisulfide -- Metabolic pathway -- Functional genes; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.126108
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Optimized S 2- /NO2 – molar ratio was 3.5 for S 0 generation and S 2-, NO2 – removal. S3 2- as an intermediate was changed to SO4 2- mainly by dsrA/B gene in biofilm. Sulfurovum and Desulfocapsa dominated activated sludge and biofilm. Abundance of sulfate-reducing bacteria increased along the reactor wall to middle. Sulfide oxidation genes showed the highest relative abundance in suspended sludge. Abstract: Types of microbial aggregates have essential effects on bacterial communities' characteristics, thus affecting the pollutants removal. An up-flow biofilm reactor was used to study the different performances of S 2- /NO2 – removal and functional genes in suspended sludge and biofilms. The metabolic pathways of sulfurous and nitrogenous pollutants in the desulfurization-denitrification process were proposed. The results showed that S 0 formation dominated the reactor with a high S 2- concentration. Autotrophic Sulfurovum responsible for S 2- /S 0 oxidation was the only dominant bacteria in suspended sludge. Heterotrophic Desulfocapsa responsible for SO4 2- reduction coexisted with Sulfurovum and dominated in biofilms. S 2- oxidation to S 0 was catalyzed via fccA/B and sqr genes in suspended sludge. S3 2- /S 0 oxidation to SO4 2- was catalyzed via dsrA/B gene in biofilms. SO4 2- and NO2 – were removed via the dissimilatory sulfate reduction and denitrification pathway, respectively. This work provides a fundamental and practical basis for optimizing suspended sludge/biofilm systems for S 2- /NO2 – removal.