Effect of manganese peroxidase on the decomposition of cellulosic components: Direct cellulolytic activity and synergistic effect with cellulase. (January 2022)

• Record Type: Journal Article
• Authors: Min, Kyoungseon; Kim, Yong Hwan; Kim, Jiye; Kim, Yunje; Gong, Gyeongtaek; Um, Youngsoon
• Is Part Of: Bioresource technology. Volume 343(2022)
• Publication Date: 2022-01
• Subjects: Manganese peroxidase -- Cellulose-decomposition -- Boosting cellulase activity; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.126138
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Previously unknown MnP activity on cellulose decomposition was demonstrated. MnP directly decomposed various cellulosic components to produce reducing sugar. MnP-driven Mn III -acetate complex seemed to be essential for cellulolytic activity. In addition, MnP boosted cellulase activity on carboxymethyl cellulose and Avicel®. The results would contribute to construct economically feasible biorefinery system. Abstract: Herein, it was unearthed that manganese peroxidase (MnP) from Phanerochaete chrysosporium, a lignin-degrading enzyme, is capable of not only directly decomposing cellulosic components but also boosting cellulase activity. MnP decomposes various cellulosic substrates (carboxymethyl cellulose, cellobiose [CMC], and Avicel®) and produces reducing sugars rather than oxidized sugars such as lactone and ketoaldolase. MnP with Mn II in acetate buffer evolves the Mn III -acetate complex functioning as a strong oxidant, and the non-specificity of Mn III -acetate enables cellulose-decomposition. The catalytic mechanism was proposed by analyzing catalytic products derived from MnP-treated cellopentaose. Notably, MnP also boosts cellulase activity on CMC and Avicel®, even considering the cellulolytic activity of MnP itself. To the best of the authors' knowledge, this is the first report demonstrating a previously unknown fungal MnP activity in cellulose-decomposition in addition to a known delignification activity. Consequently, the results provide a promising insight for further investigation of the versatility of lignin-degrading biocatalysts.