Microbial community assembly and dynamics in Granular, Fixed-Biofilm and planktonic microbiomes valorizing Long-Chain fatty acids at 20 °C. (January 2022)

• Record Type: Journal Article
• Authors: Singh, Suniti; Rinta-Kanto, Johanna M.; Lens, Piet N.L.; Kokko, Marika; Rintala, Jukka; O'Flaherty, Vincent; Ijaz, Umer Zeeshan; Collins, Gavin
• Is Part Of: Bioresource technology. Volume 343(2022)
• Publication Date: 2022-01
• Subjects: Fats, oils, and grease (FOG) -- Low-temperature anaerobic digestion -- Methanogenesis -- Microbial community assembly -- Null modelling; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.126098
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Granular, biofilm and planktonic assemblages developed from the same seed reservoir. Three assemblages forming metacommunity differed in diversity and composition. Multiple null models applied to quantify deterministic and stochastic mechanisms. Dominant, active role of acetoclastic Methanosaeta confirmed in granules and biofilm. Dynamic, non-core-microbiome taxa correlated with environmental variables. Abstract: Distinct microbial assemblages evolve in anaerobic digestion (AD) reactors to drive sequential conversions of organics to methane. The spatio-temporal development of three such assemblages (granules, biofilms, planktonic) derived from the same inoculum was studied in replicated bioreactors treating long-chain fatty acids (LCFA)-rich wastewater at 20 °C at hydraulic retention times (HRTs) of 12–72 h. We found granular, biofilm and planktonic assemblages differentiated by diversity, structure, and assembly mechanisms; demonstrating a spatial compartmentalisation of the microbiomes from the initial community reservoir. Our analysis linked abundant Methanosaeta and Syntrophaceae -affiliated taxa ( Syntrophus and uncultured ) to their putative, active roles in syntrophic LCFA bioconversion. LCFA loading rates (stearate, palmitate), and HRT, were significant drivers shaping microbial community dynamics and assembly. This study of the archaea and syntrophic bacteria actively valorising LCFAs at short HRTs and 20 °C will help uncover the microbiology underpinning anaerobic bioconversions of fats, oil and grease.