CDX-2 Protein and mRNA Expression in Canine Intestinal Adenocarcinoma. (April 2021)

• Record Type: Journal Article
• Authors: Cho, Seung-Hee; Seung, Byung-Joon; Kim, Soo-Hyeon; Bae, Min-Kyung; Lim, Ha-Young; Sur, Jung-Hyang
• Is Part Of: Journal of comparative pathology. Volume 184(2021)
• Page Start: 24
• Page End: 30
• Publication Date: 2021-04
• Subjects: canine intestinal adenocarcinoma -- CDX-2 -- immunohistochemistry -- RNA in-situ hybridization; Veterinary pathology -- Periodicals; Veterinary therapeutics -- Periodicals; Pathology, Comparative -- Periodicals; Therapeutics -- Periodicals; Pathology, Veterinary -- Periodicals; 636.089607
• DOI: 10.1016/j.jcpa.2021.01.010
• Languages: English
• ISSNs: 0021-9975

Abstract

Summary: Caudal-related homeobox transcription factor 2 ( CDX-2 ) is a specific cell marker employed in the diagnosis of human colorectal cancer. Reduced CDX-2 expression is associated with several indicators of poor prognosis in human colorectal cancer. In the present study, CDX-2 protein levels were evaluated and patterns of CDX- 2 mRNA accumulation are described for the first time in canine intestinal adenocarcinoma (CIA). Canine intestinal epithelial biopsies from 21 CIAs and 14 non-neoplastic control tissues were retrospectively evaluated for CDX-2 expression and CDX- 2 mRNA levels by immunohistochemistry and RNA in-situ hybridization (RNA-ISH), respectively. The mean percentage or intensity of expression was decreased in the CIA group ( P = 0.000). RNA-ISH demonstrated a significant correlation between the decrease in CDX- 2 mRNA levels and CDX-2 protein expression ( P = 0.000). CDX-2 downregulation, in terms of protein as well as mRNA levels, may serve as a diagnostic marker in CIA.