Comparative Immunohistochemical Analysis of IMP3, GLUT1, EMA, CD146, and Desmin for Distinguishing Malignant Mesothelioma From Reactive Mesothelial Cells. Issue 1 (1st January 2014)

• Record Type: Journal Article
• Authors: Minato, Hiroshi; Kurose, Nozomu; Fukushima, Mana; Nojima, Takayuki; Usuda, Katsuo; Sagawa, Motoyasu; Sakuma, Tsutomu; Ooi, Akishi; Matsumoto, Isao; Oda, Makoto; Arano, Yoshihiko; Shimizu, Junzo
• Is Part Of: American journal of clinical pathology. Volume 141:Issue 1(2014)
• Page Start: 85
• Page End: 93
• Publication Date: 2014-01-01
• Subjects: Malignant mesothelioma -- Reactive mesothelial cell -- Immunohistochemistry -- IMP3 -- GLUT1 -- EMA -- CD146 -- Desmin; Diagnosis, Laboratory -- Periodicals; Pathology -- Periodicals; 616.07
• DOI: 10.1309/AJCP5KNL7QTELLYI
• Languages: English
• ISSNs: 0002-9173

Abstract

Abstract: Objectives: To identify useful biomarkers for differentiating between malignant mesothelioma (MM) and reactive mesothelial cells (RMCs). Methods: Formalin-fixed, paraffin-embedded (FFPE) tissues from 34 MM and 40 RMC samples were analyzed using immunohistochemistry, and the findings were compared. Results: Positive markers for MM included insulin-like growth factor 2 messenger RNA binding protein 3 (IMP3), glucose transporter 1 (GLUT1), epithelial membrane antigen (EMA), and CD146, which showed sensitivities of 94%, 85%, 79%, and 71% and specificities of 78%, 100%, 88%, and 98%, respectively. In sarcomatoid MM, EMA had significantly lower expression than did IMP3, GLUT1, and CD146 ( P < .001). The areas under receiver operating characteristic curves were the highest for IMP3 (0.95), followed by GLUT1 (0.93). When the optimal cutoff points for IMP3 (30%) and GLUT1 (10%) were used, the sensitivity of IMP3 and GLUT1 for MM was 100%, and the specificity of both for MM was 95%. Conclusions: The combination of IMP3 and GLUT1 is most appropriate for distinguishing MM from RMC using FFPE sections.