Development of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody. Issue 1 (1st January 2018)

• Record Type: Journal Article
• Authors: Sun, Jiajia; Liu, Liqiang; Song, Shanshan; Cui, Gang; Kuang, Hua
• Is Part Of: Food and agricultural immunology. Volume 29:Issue 1(2018)
• Page Start: 930
• Page End: 940
• Publication Date: 2018-01-01
• Subjects: Capsaicinoids -- immunochromatographic strip -- monoclonal antibody -- waste oil; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2018.1490394
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: The problem of waste oil is a food safety issue that requires urgent resolution. In this study, a novel hapten was designed for the rapid detection of capsaicinoids, a marker of waste oil, and a monoclonal antibody (MAb) 5D7 was generated that is specific for three major capsaicinoids: capsaicin (CPC), dihydrocapsaicin (DCPC), and N-vanillylnonanamide (N-V). The half maximal inhibitory concentrations of MAb 5D7 for CPC, DCPC, and N-V were 1.12, 0.8, and 0.87 ng/mL, respectively. An immunochromatographic strip test based on MAb 5D7 was established for these three capsaicinoids, with visual inspection limits of <1.0 ng/mL, which satisfies the requirement for the rapid on-site inspection of waste oil.