Photoactive CO-releasing complexes containing iron – genotoxicity and ability in HO-1 gene induction in HL-60 cells. Issue 4 (22nd May 2019)

• Record Type: Journal Article
• Authors: Wysokiński, Daniel; Lewandowska, Patrycja; Zątak, Daria; Juszczak, Michał; Kluska, Magdalena; Lizińska, Daria; Rudolf, Bogna; Woźniak, Katarzyna
• Is Part Of: Toxicology research. Volume 8:Issue 4(2019)
• Page Start: 544
• Page End: 551
• Publication Date: 2019-05-22
• Subjects: Toxicology -- Periodicals; 615.9005
• DOI: 10.1039/c9tx00070d
• Languages: English
• ISSNs: 2045-452X

Abstract

Graphical Abstract: Abstract: This paper presents the results of research on the biological properties of two photoactive CO-releasing molecules containing iron, i.e. (η 5 -C5 H5 )Fe(CO)2 (η 1 - N -maleimidato) (complex A ) and (η 5 -C5 H5 )Fe(CO)2 (η 1 - N -succinimidato) (complex B ). We studied their cytotoxicity, genotoxicity and the ability of inducing the HO-1 gene in HL-60 cells. We also investigated the kinetics of DNA damage repair induced by complexes A and B . We demonstrated that complex B was not toxic to HL-60 cells in high doses (above 100 μM). The ability to induce DNA damage was higher for complex A . Importantly, there was no difference in irradiated and non-irradiated cells for both complexes. DNA damage induced by complex B was repaired efficiently, while the repair of DNA damage induced by complex A was disturbed. Complex B had a minor effect on HO-1 gene expression (less than 2-fold induction), while complex A had induced HO-1 gene expression to a great extent (over 17-fold for 10 μM) – similarly in irradiated and non-irradiated HL-60 cells. The results of our research indicate that the ability of both complexes to damage DNA and to upregulate HO-1 gene expression is not related to the release of CO. Further research is needed to test whether these compounds can be considered as potential CO carriers in humans.