TagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging. Issue 12 (11th December 2019)

• Record Type: Journal Article
• Authors: Nieves, Daniel J.; Hilzenrat, Geva; Tran, Jason; Yang, Zhengmin; MacRae, Hugh H.; Baker, Matthew A. B.; Gooding, J. Justin; Gaus, Katharina
• Is Part Of: Royal Society open science. Volume 6:Issue 12(2019)
• Publication Date: 2019-12-11
• Subjects: protein tags -- monovalent -- covalent -- DNA-PAINT imaging -- superresolution; Science -- Periodicals; 500
• DOI: 10.1098/rsos.191268
• Languages: English
• ISSNs: 2054-5703

Abstract

Abstract : Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.