Transcription closed and open complex formation coordinate expression of genes with a shared promoter region. Issue 161 (24th December 2019)

• Record Type: Journal Article
• Authors: Häkkinen, Antti; Oliveira, Samuel M. D.; Neeli-Venkata, Ramakanth; Ribeiro, Andre S.
• Is Part Of: Journal of the Royal Society interface. Volume 16:Issue 161(2019)
• Publication Date: 2019-12-24
• Subjects: transcription -- gene expression noise -- bidirectional promoter; Physical sciences -- Research -- Periodicals; Life sciences -- Research -- Periodicals; Interdisciplinary research -- Periodicals; 570.5
• DOI: 10.1098/rsif.2019.0507
• Languages: English
• ISSNs: 1742-5689

Abstract

Abstract : Many genes are spaced closely, allowing coordination without explicit control through shared regulatory elements and molecular interactions. We study the dynamics of a stochastic model of a gene-pair in a head-to-head configuration, sharing promoter elements, which accounts for the rate-limiting steps in transcription initiation. We find that only in specific regions of the parameter space of the rate-limiting steps is orderly coexpression exhibited, suggesting that successful cooperation between closely spaced genes requires the coevolution of compatible rate-limiting step configuration. The model predictions are validated using in vivo single-cell, single-RNA measurements of the dynamics of pairs of genes sharing promoter elements. Our results suggest that, in E. coli, the kinetics of the rate-limiting steps in active transcription can play a central role in shaping the dynamics of gene-pairs sharing promoter elements.