Impact of Sterilization Methods on the Seeding Ability of Human Tau Proteopathic Seeds. Issue 10 (8th September 2021)

• Record Type: Journal Article
• Authors: Fernandes, Analiese R; Dujardin, Simon; Maté de Gérando, Anastasie; Hyman, Bradley T; Frosch, Matthew P
• Is Part Of: Journal of neuropathology and experimental neurology. Volume 80:Issue 10(2021)
• Page Start: 912
• Page End: 921
• Publication Date: 2021-09-08
• Subjects: Alzheimer disease -- Safety standards -- Sterilization -- Tau -- Tauopathies -- Transmissibility; Neurology -- Diseases -- Periodicals; Neurology -- Diseases -- Physiopathology -- Periodicals; 616.8047
• DOI: 10.1093/jnen/nlab087
• Languages: English
• ISSNs: 0022-3069

Abstract

Abstract: The protein tau, when misfolded in neurodegenerative diseases, has several prion-like properties including being able to spread by cell-to-cell transfer, induce templated seeding, and exist in distinct conformational strains. These properties of transmission may present health hazards when lesion-containing biospecimens are used in research and neuropathology laboratories. We evaluated the impact standard sterilization and cleaning methods have on the capacity of tau seeds to induce aggregation. We employed a previously developed, highly sensitive FRET-based biosensor assay to assess remnant tau seeding after exposure to these procedures. For tau species derived from human Alzheimer disease tissue (brain homogenate and sarkosyl-insoluble fibrils), both autoclaving and incubation in 90.6% formic acid were sufficient to reduce tau bioactivity. By contrast, boiling was not always effective in completely blocking bioactivity in the seeding assay. Notably, only formic acid incubation was able to produce a similar reduction in tissue from a P301L mutant tau mouse model of tauopathy. Our study highlights nuances in methods for inactivation of tau seeding which may support adapted tissue processing procedures, especially in research settings. These findings also highlight the importance of universal precautions when handling human neuropathological and research laboratory materials.