Changes in chemical components and antitumor activity during the heating process of Fructus Arctii. (1st January 2019)

• Record Type: Journal Article
• Authors: Hu, Jing; Shi, Yun; Yang, Bing; Dong, Zibo; Si, Xinxin; Qin, Kunming
• Is Part Of: Pharmaceutical biology. Volume 57:Number 1(2019)
• Page Start: 363
• Page End: 368
• Publication Date: 2019-01-01
• Subjects: Traditional Chinese medicine -- stir-heating -- multi-component -- compatibility; Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.1080/13880209.2019.1616778
• Languages: English
• ISSNs: 1388-0209

Abstract

Abstract: Context: The dried fruits of Arctium lappa L. have been used in two forms in traditional Chinese medicine; crude and stir-heating Fructus Arctii. However, its processed product possesses better activity. Objective: In this study, the chemical constituents of both crude and processed Fructus Arctii and their antiproliferative activities were evaluated. Materials and methods: The seven main active components in crude and various processed Fructus Arctii were quantitatively determined using high-performance liquid chromatography (HPLC). According to the actual amount in crude and five processed samples, seven single components were combined as multi-component combinations with six different proportions. The antiproliferative activities of these compatibility component groups were examined using the CCK-8 assay. Results: During the heating process, the proportion of the seven main components changed dynamically. The contents of 3-caffeoylquinic acid (3-CQA), 3, 5-dicaffeoylquinic acid (3, 5-diCQA), and arctiin (ARC) declined, while the contents of 4-caffeoylquinic acid (4-CQA), 3, 4-dicaffeoylquinic acid (3, 4-diCQA), 4, 5-dicaffeoylquinic acid (4, 5-diCQA), and arctigenin (ARG) increased very significantly. Discussion and conclusions: The results also indicated that seven components in the processed samples had higher cytotoxic profiles against HL-60 cells than those in the crude sample. Therefore, the heating process may enhance the antitumor activity of Fructus Arctii by changing the proportion of active components.