Design of highly selective, and sensitive screen-printed electrochemical sensor for detection of uric acid with uricase immobilized polycaprolactone/polyethylene imine electrospun nanofiber. (20th January 2023)

• Record Type: Journal Article
• Authors: Muhammad, Fakhriy; Dik, Gamze; Kolak, Seda; Gedik, Kübra Karadaş; Bakar, Büşra; Ulu, Ahmet; Ateş, Burhan
• Is Part Of: Electrochimica acta. Volume 439(2023)
• Publication Date: 2023-01-20
• Subjects: Electrospun nanofiber -- Uricase -- Enzyme immobilization -- Quantum dot screen printed electrode -- Differential pulse voltammetry -- Uric acid -- Biosensor; UA Uric acid -- PCL Polycaprolactone -- PEI Polyethylene imine -- UOx Uricase -- QD SPCE Quantum dot screen printed electrode -- MB Methylene blue -- CV Cyclic voltammetry -- DPV Differential pulse voltammetry -- EIS Electrochemical impedance spectroscopy -- LoD Limits of detection -- HPLC High-performance liquid chromatography -- GC Gas chromatography -- CdSe Cadmium selenium -- FTIR Fourier transform infrared spectroscopy -- SEM Scanning electron microscopy -- XRD X-ray diffraction -- EDX Energy-dispersive X-ray spectroscopy -- PBS Phosphate-buffered saline -- ECM Equivalent circuit model -- LoQ Limits of quantification; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2022.141675
• Languages: English
• ISSNs: 0013-4686

Abstract

Highlights: Uricase immobilized PCL/PEI electrospun nanofibers were firstly fabricated. Sensor based on electrospun nanofiber exhibited promising electrochemical performance. The fabricated sensor was capable of sensing in a wide working range (5–52 µM). This sensor exhibited good repeatability and stability with low LoD of 1.85 µM. This sensor was successfully employed to detect uric acid in rat blood serum. Abstract: Uric acid (UA) plays a significant role in nerve center, human metabolism, and kidney system. Therefore, it is necessary to establish a simple, rapid, and sensitive detection method for UA . In recent years, researchers have been highly attracted by nanomaterials with satisfactory functions in electrochemical applications. In this study, polycaprolactone (PCL)/polyethylene imine (PEI) nanofiber membranes were prepared and used for the immobilization of uricase (UOx). The prepared membranes were characterized in terms of structure, composition, and morphology. Afterward, the quantum dot screen printed electrode (QD SPCE) was modified with PCL/PEI nanofiber membranes with and without methylene blue (MB) as an electron mediator. The electrochemical performance of the developed sensors was investigated by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). Under the optimal conditions, the modified sensors provided a broad linear range (5.0–52.0 µM) for the electrochemical detection of UA. Limit of detection (LoD) values were determined as 3.96 µM and 1.85 µM for the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/QD SPCE, respectively. The four-week stability results showed the change in current for the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/QD SPCE to be 92% and 87%, respectively. Additionally, in the mixed interference test remaining current ratios for UA were 95% and 82% for the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/QD SPCE, respectively. Most importantly, the effectiveness of the electrodes was also verified in real sample detection with satisfactory recovery (∼98-112%). Overall, the results showed that the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/QD SPCE have the advantages of simplicity, high sensitivity, and good selectivity for UA determination. Graphical Abstract: Image, graphical abstract