Virucidal activity in vitro of mouthwashes against a feline coronavirus type II. (12th November 2021)

• Record Type: Journal Article
• Authors: Buonavoglia, Alessio; Camero, Michele; Lanave, Gianvito; Catella, Cristiana; Trombetta, Claudia Maria; Gandolfi, Maria Giovanna; Palazzo, Gerardo; Martella, Vito; Prati, Carlo
• Is Part Of: Oral diseases. Volume 28(2022)Supplement 2
• Page Start: 2492
• Page End: 2499
• Publication Date: 2021-11-12
• Subjects: dental practice -- feline coronavirus -- in vitro -- mouthwashes -- virucidal activity; Mouth -- Diseases -- Research -- Periodicals; 617.522
• DOI: 10.1111/odi.14067
• Languages: English
• ISSNs: 1354-523X

Abstract

Abstract: Transmission of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) can occur through saliva and aerosol droplets deriving from the upper aerodigestive tract during coughing, sneezing, talking, and even during oral inspection or dental procedures. The aim of this study was to assess in vitro virucidal activity of commercial and experimental mouthwashes against a feline coronavirus (FCoV) strain. Commercial and experimental (commercial‐based products with addition of either sodium dodecyl sulfate (SDS) or thymus vulgaris essential oil (TEO) at different concentrations) mouthwashes were placed in contact with FCoV for different time intervals, that is, 30 s (T30), 60 s (T60), and 180 s (T180); subsequently, the virus was titrated on Crandell Reese Feline Kidney cells. An SDS‐based commercial mouthwash reduced the viral load by 5 log10 tissue culture infectious dose (TCID)50 /50 µl at T30 while a cetylpyridinium (CPC)‐based commercial mouthwash was able to reduce the viral titer of 4.75 log10 at T60. Furthermore, five experimental mouthwashes supplemented with SDS reduced the viral titer by 4.75–5 log10 according to a dose‐ (up to 4 mM) and time‐dependent fashion.