Novel sequence types of extended-spectrum and acquired AmpC beta-lactamase producing Escherichia coli and Escherichia clade V isolated from wild mammals. Issue 8 (24th July 2017)

• Record Type: Journal Article
• Authors: Alonso, Carla Andrea; Alcalá, Leticia; Simón, Carmen; Torres, Carmen
• Is Part Of: FEMS microbiology ecology. Volume 93:Issue 8(2017:Aug.)
• Publication Date: 2017-07-24
• Subjects: ESBL -- AmpC -- cryptic Escherichia clade -- antimicrobial resistance -- wildlife -- Spain; Microbial ecology -- Periodicals; Microbiology -- Periodicals; 579.17
• DOI: 10.1093/femsec/fix097
• Languages: English
• ISSNs: 0168-6496

Abstract

Abstract: The closer contact with wildlife due to the growing human population and the destruction of natural habitats emphasizes the need of gaining insight into the role of animals as source of antimicrobial resistance. Here, we aim at characterizing the antimicrobial resistance genes and phylogenetic distribution of commensal Escherichia coli from 62 wild mammals. Isolates exhibiting resistance to ≥1 antibiotic were detected in 25.8% of the animals and 6.4% carried an extended-spectrum beta-lactamase (ESBL)/AmpC-producing E. coli . Genetic mechanisms involved in third-generation cephalosporin resistance were as follows: (i) hyperproduction of chromosomal AmpC (hedgehog), (ii) production of acquired CMY-2 β-lactamase (hedgehog), (iii) production of SHV-12 and CTX-M-14 ESBLs (n = 2, mink and roe-deer). ESBL genes were transferable by conjugation, and bla CMY-2 was mobilized by a 95kb IncI1 plasmid. The distribution of the phylogenetic groups in the E. coli collection studied was B1 (44.6%), B2 (24.6%), E (15.4%), A (4.6%) and F (3.1%). Five isolates (7.7%) were cryptic Escherichia clades (clade IV, 4 mice; clade V, 1 mink). ESBL/AmpC- E. coli isolates showed different sequence types (STs): ST1128/B1, ST4564/B1 (new), ST4996/B1 (new) and a non-registered ST. This study contributes to better understand the E. coli population and antimicrobial resistance flow in wildlife and reports new AmpC- E. coli STs and a first described ESBL-producing Escherichia clade V isolate. Abstract : Novel sequence types among Escherichia coli isolates exhibiting AmpC phenotype and a first described ESBL-producing cryptic Escherichia clade V have been identified in wild mammals, feces, which underline the undesirable consequences of their potential entry in the community or clinical settings.