Characterization of a lactose-responsive promoter of ATP-binding cassette (ABC) transporter gene from Lactobacillus acidophilus 05–172. Issue 16 (2nd August 2017)

• Record Type: Journal Article
• Authors: Zeng, Zhu; Zuo, Fanglei; Yu, Rui; Zhang, Bo; Ma, Huiqin; Chen, Shangwu
• Is Part Of: FEMS microbiology letters. Volume 364:Issue 16(2017)
• Publication Date: 2017-08-02
• Subjects: Lactobacillus -- lactose induction -- promoter -- expression vector -- heterologous expression; Microbiology -- Periodicals; 579
• DOI: 10.1093/femsle/fnx167
• Languages: English
• ISSNs: 0378-1097

Abstract

Abstract: A novel lactose-responsive promoter of the ATP-binding cassette (ABC) transporter gene Lba1680 of Lactobacillus acidophilus strain 05–172 isolated from a traditionally fermented dairy product koumiss was characterized. In L. acidophilus 05–172, expression of Lba1680 was induced by lactose, with lactose-induced transcription of Lba1680 being 6.1-fold higher than that induced by glucose. This is in contrast to L. acidophilus NCFM, a strain isolated from human feces, in which expression of Lba1680 and Lba1679 is induced by glucose. Both gene expression and enzyme activity assays in L. paracasei transformed with a vector containing the inducible Lba1680 promoter (P L b a1680 ) of strain 05–172 and a heme-dependent catalase gene as reporter confirmed that P Lba1680 is specifically induced by lactose. Its regulatory expression could not be repressed by glucose, and was independent of cAMP receptor protein. This lactose-responsive promoter might be used in the expression of functional genes in L. paracasei incorporated into a lactose-rich environment, such as dairy products. Abstract : A novel lactose-responsive promoter of an ATP-binding cassette (ABC) transporter gene from Lactobacillus acidophilus was characterized.