Regulation of TGF-β-mediated endothelial-mesenchymal transition by microRNA-27. (13th March 2017)

• Record Type: Journal Article
• Authors: Suzuki, Hiroshi I.; Katsura, Akihiro; Mihira, Hajime; Horie, Masafumi; Saito, Akira; Miyazono, Kohei
• Is Part Of: Journal of biochemistry. Volume 161:Number 5(2017:May)
• Page Start: 417
• Page End: 420
• Publication Date: 2017-03-13
• Subjects: TGF-β -- EndMT -- microRNA -- α-SMA -- Elk1; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Electronic journals; 572.05
• DOI: 10.1093/jb/mvx017
• Languages: English
• ISSNs: 0021-924X

Abstract

Abstract : Multiple microRNAs (miRNAs) regulate epithelial-mesenchymal transition and endothelial-mesenchymal transition (EndMT). Here we report that microRNA-27b (miR-27b) positively regulates transforming growth factor-β (TGF-β)-induced EndMT of MS-1 mouse pancreatic microvascular endothelial cells. TGF-β induced miR-23b/24-1/27b expression, and inhibition of miR-27 suppressed TGF-β-mediated induction of mesenchymal genes. Genome-wide miRNA target analysis revealed that miR-27 targets Elk1, which acts as a competitive inhibitor of myocardin-related transcription factor-serum response factor signalling and as a myogenic repressor. miR-27b was also found to regulate several semaphorin receptors including Neuropilin 2, Plexin A2 and Plexin D1. These results suggest important roles of miR-27 in TGF-β-driven EndMT.