First detection and complete genome analysis of porcine circovirus‐like virus P1 and porcine circovirus‐2 in yak in China. Issue 6 (1st September 2022)

• Record Type: Journal Article
• Authors: Zhu, Jiaping; Xiao, Qi; Wen, Libin; Yin, Lihong; Zhang, Fengxi; Li, Tianjiao; Banma, Zelang; He, Kongwang; Suolang, Sizhu
• Is Part Of: Veterinary medicine and science. Volume 8:Issue 6(2022)
• Page Start: 2553
• Page End: 2561
• Publication Date: 2022-09-01
• Subjects: phylogeny -- porcine circovirus‐like virus P1 -- porcine circovirus‐2 -- sequencing -- yak; Veterinary medicine -- Periodicals; Animal Diseases; Veterinary medicine; Periodicals; Periodicals; 636.08905
• DOI: 10.1002/vms3.911
• Languages: English
• ISSNs: 2053-1095

Abstract

Abstract: Porcine circovirus‐like virus P1, like porcine circovirus type 2 (PCV2), is a potential pathogen of post‐weaning multisystemic wasting syndrome in swine. Yaks are a valuable species and an iconic symbol of the Tibet Plateau which is the highest and largest plateau in the world. In this study, a total of 105 yak diarrheal samples, collected from 13 farms in Linzhi in the Tibet Plateau from January 2019 to December 2021, that were screened for P1 and PCV2 by polymerase chain reaction, 10.48% ( n = 11) were positive for P1, 4.76% ( n = 5) for PCV2, and 5.71% ( n = 6) were positive for coinfection of P1 and PCV2. In addition, the whole genomes of eight P1 strains and eight PCV2 strains were sequenced. Alignment of deduced amino acid sequences of P1 ORF1 and PCV2 ORF2 gene revealed that ON012566 had one unique amino acid mutation at residues 137 (T to P). This mutation has important implication for the study of virus virulence, tissue tropism, and immune response. Phylogenetic analysis shows that the yak‐origin P1 strains in this study with cattle‐origin P1 reference strains were grouped into one cluster. The yak‐origin PCV2 (ON012566) and a buffalo‐origin PCV2 (KM116514) reference strain clustered in the same branch in the PCV2b regions. Meanwhile, the remaining PCV2 strains and buffalo‐origin PCV2 reference strain (ON012565) clustered in the PCV2d regions. To summarize, to our knowledge, this is the first report on the molecular prevalence and genome characteristics of P1 and PCV2 in yaks in the world and will contribute to further study of the molecular epidemiology, source, and evolution of P1 and PCV2 strains. Abstract : Porcine circovirus‐like virus P1, like Porcine circovirus type 2, is a potential pathogen of post‐weaning multisystemic wasting syndrome (PMWS) in swine. P1 or PCV2 has a risk of cross‐species transmission. P1 has high homology with PCV1 and PCV2 and both PCV1 and PCV2 can be detected in human samples. For the sake of public health and safety, we should attach importance to the research of cross‐species transmission of P1 and PCV2. Yaks are a valuable species and an iconic symbol of the Tibet Plateau, which is the highest and largest plateau in the world. The research on the genetic evolution of the virus in such a special environment is of great significance. To the best of our knowledge, this is the first report of circulation of PCV2 and P1 among yaks in the world.