Overexpression of PdeGATA3 results in a dwarf phenotype in poplar by promoting the expression of PdeSTM and altering the content of gibberellins. (6th August 2022)

• Record Type: Journal Article
• Authors: Liu, Meifeng; Huang, Liyu; Zhang, Yan; Yan, Zhaogui; Wang, Nian
• Editors: David-Schwartz, Rakefet
• Is Part Of: Tree physiology. Volume 42:Number 12(2022)
• Page Start: 2614
• Page End: 2626
• Publication Date: 2022-08-06
• Subjects: dwarf -- GA20 oxidase (GA20ox) -- GATA transcription factor (TF) -- gibberellins -- internode length -- petiole length -- Populus -- SHOOTMERISTEMLESS (STM); Trees -- Physiology -- Periodicals; 582.16
• DOI: 10.1093/treephys/tpac086
• Languages: English
• ISSNs: 0829-318X

Abstract

Abstract: In previous studies, GA20 oxidase (GA20ox) has been identified to be an important enzyme in the biosynthesis of GA, and SHOOTMERISTEMLESS (STM) can repress the expression of GA20ox . In this study, the GATA transcription factor PdeGATA3 was identified in the poplar line NL895, and its overexpression (OE) transgenic lines showed a dwarf phenotype. RNA sequencing (RNA-Seq) analysis suggested that OE PdeGATA3 could promote the expression of PdeSTM and repress the expression of PdeGA20ox . Therefore, we hypothesized that PdeGATA3 would directly promote the expression of PdeSTM and that PdeSTM would repress the expression of PdeGA20ox . Four experiments, a dual-luciferase reporter assay, GUS transient coexpression assay, yeast one-hybrid assay and electrophoretic mobility shift assay, were conducted and verified that PdeGATA3 could promote the expression of PdeSTM by binding GATA-Boxes in its promoter. OE PdeSTM in poplar resulted in a dwarf phenotype and repressed the expression of PdeGA20ox . GA measurement of the OE PdeSTM and PdeGATA3 lines showed that GA3 and GA4 contents were significantly lower than those in the wild type (WT). Accordingly, we put forward a regulation model involving plant height regulation by PdeGATA3, PdeSTM and PdeGA20ox .