PARK7/DJ-1 inhibition decreases invasion and proliferation of uveal melanoma cells. Issue 1 (February 2023)

• Record Type: Journal Article
• Authors: Lago-Baameiro, Nerea; Santiago-Varela, Maria; Camino, Tamara; Silva-Rodríguez, Paula; Bande, Manuel; Blanco-Teijeiro, Maria Jose; Pardo, Maria; Piñeiro, Antonio
• Is Part Of: Tumori. Volume 109:Issue 1(2023)
• Page Start: 47
• Page End: 53
• Publication Date: 2023-02
• Subjects: Uveal melanoma -- DJ-1 -- PARK7 -- biomarker; Cancer -- Periodicals; 616.994
• DOI: 10.1177/03008916211061766
• Languages: English
• ISSNs: 0300-8916

Abstract

Introduction: PARK7/DJ-1 is an oncogene that is associated with tumorigenesis in many cancers. Recent studies have demonstrated the importance of DJ-1 in the origin and development of uveal melanoma (UM). We present an analysis of the role of the DJ-1 protein in UM cells, especially in its effect on proliferation and migration. Methods: UM cells from a primary tumor, Mel 270, and its liver metastasis, OMM2.5, were transfected with lentiviral-delivered shRNA against PARK7/DJ-1. Evaluation of cell migration and proliferation was performed using the xCELLigence real-time cell analyzer (RTCA). The effect of DJ-1 inhibition on the PTEN-Akt signaling pathway was also studied by immunoblotting. Results: The silencing of PARK7/DJ-1 oncoprotein expression produced a significant decrease of phosphorylated Akt (S473) in Mel270 and in metastatic OMM2.5 UM cells with no alteration on tumor suppressor PTEN expression. The diminution of PARK7/DJ-1 expression significantly inhibited real-time proliferation and invasion of Mel270 and OMM2.5 and the invasion potential of the metastatic cells. Conclusion: DJ-1 appears to play a key role on the PTEN/Akt pathway in UM. DJ-1 inhibition appears to have a negative effect on proliferation and invasion of UM cells. This suggests DJ-1 as a potential therapeutic target in UM.