Molecular data reveal a new species of Seuratascaris Sprent, 1985 (Nematoda: Ascaridoidea) from Quasipaa exilispinosa (Liu & Hu) (Amphibia: Anura). (April 2023)

• Record Type: Journal Article
• Authors: Chen, Hui-Xia; Li, Liang
• Is Part Of: Parasitology international. Volume 93(2023)
• Publication Date: 2023-04
• Subjects: Parasite -- Nematode -- Amphibia -- Integrated taxonomy -- Genetic data -- Species diversity; Parasitology -- Periodicals; Parasites -- Periodicals; Parasitic Diseases -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; 571.99905
• DOI: 10.1016/j.parint.2022.102698
• Languages: English
• ISSNs: 1383-5769

Abstract

Abstract: The genus Seuratascaris Sprent, 1985 is a group of obligate nematode parasites of amphibians. In the present study, a new species of Seuratascaris, S. physalis sp. n. was described using light and scanning electron microscopy based on specimens collected from Quasipaa exilispinosa (Liu & Hu) (Amphibia: Anura) in China. The new species differs from S. numidica (Seurat, 1917) by the cuticle of the cervical region distinctly inflated to form a cephalic vesicle-like structure and the absence of single medio-ventral precloacal papilla. The molecular characterization of the nuclear large ribosomal DNA (28S) and internal transcribed spacer (ITS) and the mitochondrial cytochrome c oxidase subunit 1 ( cox 1), cytochrome c oxidase subunit 2 ( cox 2) and 12S small subunit ribosomal RNA gene of S. physalis sp. n., together with the 28S, cox 2 and 12S of S. numidica are provided for the first time. Molecular analysis revealed the presence of high level of interspecific genetic variation between the two species in the ITS (5.50%), cox 1 (13.3%), cox 2 (10.6%) and 12S regions (10.5%), which strongly supported that S. physalis sp. n. represented a different species from S. numidica . Angusticaecum ranae Wang, Zhao & Chen, 1978 reported from the frog Quasipaa spinosa (David) (Anura: Dicroglossidae) in China was transferred into the genus Seuratascaris as S. ranae (Wang, Zhao & Chen, 1978) comb. n. based on the morphology of lips and the presence of very short and robust spicules without alae and small numbers of precloacal papillae. The present study provided useful genetic data for molecular identification of species of Seuratascaris and provides the foundation for being able to determine if S. numidica represents a species complex of some sibling species or a single species. Graphical abstract: Unlabelled Image Highlights: S. physalis sp. n. was described using LM and SEM. High level of interspecific genetic variation detected in different genetic markers. Molecular evidence supported S. physalis sp. n. to be different from S. numidica .