Liquid hot water pretreatment combined with high-solids enzymatic hydrolysis and fed-batch fermentation for succinic acid sustainable processed from sugarcane bagasse. (February 2023)

• Record Type: Journal Article
• Authors: Xu, Chao; Xiong, Yaru; Zhang, Jun; Li, Kuntai; Zhong, Saiyi; Huang, Shushi; Xie, Chunliang; Gong, Wenbing; Zhu, Zuohua; Zhou, Yingjun; Peng, Yuande
• Is Part Of: Bioresource technology. Volume 369(2023)
• Publication Date: 2023-02
• Subjects: Liquid hot water -- Sugarcane bagasse -- Enzymatic hydrolysis -- Succinic acid -- Fermentation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.128389
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Realized efficient high-solids enzymatic hydrolysis of unwashed sugarcane bagasse. Achieved pretreatment liquid emission reduction and C5/C6 sugars full utilization. Pretreatment, enzymatic hydrolysis and fermentation procedures were coupled and optimized. Established sustainable process for biorefining succinic acid from sugarcane bagasse. Abstract: In order to sustainable process of bio-succinic acid (SA), response surface methodology (RSM) was applied to optimize liquid hot water pretreatment pretreatment of sugarcane bagasse (SCB), followed by high-solids enzymatic hydrolysis of pretreated residual that without washing, then the hydrolysates and partial pretreatment liquid were used as carbon sources for SA fermentation. Results showed that the highest sugars yield could be achieved at pretreatment conditions of temperature 186 °C, time 25 min and solid-to-liquid ratio 0.08; enzymatic digestion the pretreated residuals at 20 % (w/v) solid content via enzymes reconstruction and fed-batch strategy, the obtained sugars reached to 121 g/L; by controlling the nutrition and conditions of the fermentation process, most of the C5 and C6 sugars in the hydrolysate and pretreatment liquid were converted into SA with a conversion rate high to 280 mg/g SCB. This study can provide a novel clue for clean and efficient biorefining of chemicals.