Synthesis of temperature and pH responsive lignin-grafted sulfobetaine for efficiently recycling cellulase. (February 2023)

• Record Type: Journal Article
• Authors: Li, Feiyun; Pang, Yuxia; Lou, Hongming; Qiu, Xueqing
• Is Part Of: Bioresource technology. Volume 369(2023)
• Publication Date: 2023-02
• Subjects: Lignin -- Cellulase recovery -- UCST response -- pH response -- Enzymatic hydrolysis; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.128357
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Cellulase was recycled by using pH-UCST-type LSB, and the process is sustainable while realizing the value-added utilization of industrial lignin. Highlights: LSB showed a pH-UCST-sensitive response and was recyclable. Cellulase was efficiently recycled at higher pH by using LSB. The value-added utilization of enzymatic hydrolysis lignin was realized. Abstract: Recycling cellulase can reduce the cost of lignocellulosic enzymatic hydrolysis. Here, a lignin-grafted sulfobetaine (LSB) was first synthesized by grafting sulfobetaine (SB) on enzymatic hydrolysis lignin (EHL). LSB had a sensitive response of pH and temperature. LSB dissolved under the conditions of lignocellulosic enzymatic hydrolysis (pH 5.0, 50 °C). After hydrolysis, LSB co-precipitated with cellulase when lowering pH of the hydrolysate to 4.0 and cooling to 25 °C. When 3.0 g/L LSB-100 was added to the hydrolysis system of corncob residue (CCR), 70 % of amount of cellulase was saved. LSB had a remarkable response and stronger cellulase recovery capacity. This was attributed that carboxylate radical in LSB was protonated, and positive and negative ions of SB associated to form salt at 25 °C. This work provides a new idea for reducing the cost for preparing fermentable sugars from lignocellulose, and increasing the added value of EHL.