Metagenomic insights into role of red mud in regulating fate of compost antibiotic resistance genes mediated by both direct and indirect ways. (15th January 2023)

• Record Type: Journal Article
• Authors: Huang, Yite; Wen, Xiaoli; Li, Jixuan; Niu, Qiuqi; Tang, Aixing; Li, Qunliang
• Is Part Of: Environmental pollution. Volume 317(2023)
• Publication Date: 2023-01-15
• Subjects: Composting -- Antibiotics -- Mobile genetic elements -- Quorum sensing -- Network analysis; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2022.120795
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: In this study, the amendment of red mud (RM) in dairy manure composting on the fate of antibiotic resistance genes (ARGs) by both direct (bacteria community, mobile genetic elements and quorum sensing) and indirect ways (environmental factors and antibiotics) was analyzed. The results showed that RM reduced the total relative abundances of 10 ARGs and 4 mobile genetic elements (MGEs). And the relative abundances of total ARGs and MGEs decreased by 53.48% and 22.30% in T (with RM added) on day 47 compared with day 0. Meanwhile, the modification of RM significantly increased the abundance of lsr K, pvd Q and ahl D in quorum quenching (QQ) and decreased the abundance of lux S in quorum sensing (QS) (P < 0.05), thereby attenuating the intercellular genes frequency of communication. The microbial community and network analysis showed that 25 potential hosts of ARGs were mainly related to Firmicutes, Proteobacteria and Actinobacteria . Redundancy analysis (RDA) and structural equation model (SEM) further indicated that RM altered microbial community structure by regulating antibiotic content and environmental factors (temperature, pH, moisture content and organic matter content), which then affected horizontal gene transfer (HGT) in ARGs mediated by QS and MGEs. These results provide new insights into the dissemination mechanism and removal of ARGs in composting process. Graphical abstract: Image 1 Highlights: Red mud reduced the concentration of residual antibiotics in composting. The abundances of ARGs and MGEs were decreased by 53.48% and 22.30% with red mud. Proteobacteria was the main contributor to ARGs and MGEs through network analysis. The dissemination of ARGs was affected by red mud in both direct and indirect ways.