A review of recent advances in plant-pathogen detection systems. Issue 12 (December 2022)

• Record Type: Journal Article
• Authors: Patel, Rhea; Mitra, Bappa; Vinchurkar, Madhuri; Adami, Andrea; Patkar, Rajul; Giacomozzi, Flavio; Lorenzelli, Leandro; Baghini, Maryam Shojaei
• Is Part Of: Heliyon. Volume 8:Issue 12(2022)
• Publication Date: 2022-12
• Subjects: Agriculture -- Pathogen detection -- Miniaturized systems -- Biosensors; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2022.e11855
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: Worldwide, a substantial economic loss in agricultural products is caused by plant pathogens. The increased losses in agriculture have drawn attention towards the development of miniaturized pathogen detection systems for phytopathology. This review paper's main selling point supports recent research (from 2015 to 2022) and technological advancements in the field of plant pathogen detection. The article discusses in depth important developments in the loop-mediated isothermal amplification (LAMP) assay, microfluidics, Molecular Imprinted Polymer (MIP) based biosensors, digital droplet PCR (ddPCR), disposable all-printed electronics, and nanoparticle-based sensors for instantaneous pathogen detection in agricultural applications. Utilizing nanoparticles to identify agricultural pathogens is a crucial topic that is explored. A brief on various commercially available detection systems worldwide have been listed. Finally, we discuss the perspective in the development of portable miniaturized systems and novel assay technologies based on advanced nanomaterials. Gold standard techniques: Although Polymerase Chain Reaction (PCR) and culture counting have been widely used for plant pathogen detection, they are not appropriate for measurements made in the field due to their higher installation costs, lack of portability, need for well-equipped laboratories, and requirement of skilled personnel. Therefore, these recent trends are overtaking the traditional methods in Agri-diagnostics because of their superior performances and suitability for the task. Highlights: It's critical to identify plant diseases early to avert financial losses. Traditional methods have limitations. Recent advances in plant pathogen detection system. Application of nanotechnology in biosensing system. Commercially available devices for plant disease detection. Abstract : Agriculture; Pathogen detection; Miniaturized systems; Biosensors.