Energy-transfer sensitization of BODIPY fluorescence in a dyad with a two-photon absorbing antenna chromophore. (February 2023)

• Record Type: Journal Article
• Authors: Dominguez-Alfaro, Antonio; Pais, Vânia F.; Guzmán-Ríos, David B.; Collado, Daniel; Nájera, Francisco; Pérez-Inestrosa, Ezequiel; Pischel, Uwe
• Is Part Of: Dyes and pigments. Volume 210(2023)
• Publication Date: 2023-02
• Subjects: BODIPY dyes -- Energy transfer -- Electron transfer -- Two-photon absorption -- Bioimaging; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2022.110950
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: A bichromophoric dyad composed of a Prodan-derived moiety and a BODIPY dye was prepared, photophysically characterized, and tested for multiphoton fluorescence microscopy of N13 microglial cells. The dyad shows highly efficient energy transfer, independent on the solvent polarity of the medium. However, in polar media photoinduced electron transfer is a competitive pathway. The Prodan-derived chromophore shows significant two-photon absorption. Hence, it can be used a two-photon-absorbing antenna for the sensitization of BODIPY fluorescence. Graphical abstract: Image 1 Highlights: A BODIPY-Prodan dyad with a light-harvesting antenna and a non-conjugating linker was synthesized. Highly efficient two-photon-induced energy transfer mediates the excited state communication between the two chromophores. Intramolecular photoinduced electron transfer is controlled by solvent polarity. Two-photon excitation under conditions of the imaging of N13 microglial cells is demonstrated.