Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip (Small 51/2022). Issue 51 (22nd December 2022)

• Record Type: Journal Article
• Authors: Kalyana Sundaram, Ramalingam Venkat; Bera, Manindra; Coleman, Jeff; Weerakkody, Jonathan S.; Krishnakumar, Shyam S.; Ramakrishnan, Sathish
• Is Part Of: Small. Volume 18:Issue 51(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-12-22
• Subjects: asymmetric lipid membranes -- native suspended plasma membranes -- protein diffusion -- protein fluorescent resonant energy transfer (FRET) -- silicon microarrays -- single molecule counting -- total internal reflection fluorescence penetration depth; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202270277
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract : Single‐Molecule Investigations In article number 2205567, Sathish Ramakrishnan and co‐workers present a tunable silicon‐based platform that supports suspended lipid membranes (SLIM) to interrogate membrane protein assembly and dynamics using total internal reflection fluorescence (TIRF) microscopy. By generating bilayers from giant plasma membrane vesicles derived from different cell types, it is possible to capture fast dynamics of protein function on the inner leaflet of cells while retaining native lipid architecture and asymmetry.