Intravital Imaging of Hepatic Blood Biliary Barrier in Live Mice. Issue 10 (5th October 2021)

• Record Type: Journal Article
• Authors: Vats, Ravi; Kaminski, Tomasz W.; Pradhan‐Sundd, Tirthadipa
• Is Part Of: Current protocols. Volume 1:Issue 10(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-10-05
• Subjects: bile flow -- blood bile barrier -- intravital imaging -- live liver imaging -- two‐photon microscopy; Life sciences -- Laboratory manuals -- Periodicals; Biology -- Laboratory manuals -- Periodicals; Life sciences -- Technique -- Periodicals; Biology -- Technique -- Periodicals; 570.028
• DOI: 10.1002/cpz1.256
• Languages: English
• ISSNs: 2691-1299

Abstract

Abstract: Understanding the kinetics and spatiotemporal interactions of living cells within the tissue environment requires real‐time imaging. The introduction of two‐photon microscopy has substantially boosted the power of live intravital imaging, making it possible to obtain information of individual cells in near‐physiologic conditions within intact tissues nondestructively. Intravital imaging of the liver has proved useful in understanding its 3D structure, function, and dynamic cellular interactions. Recently we have shown that integrity of the blood‒bile barrier in different physiologic and pathophysiologic conditions can be imaged in real time using intravital microscopy. Here we discuss the real‐time intravital imaging method to visualize blood‒bile barrier integrity in the murine liver. © 2021 Wiley Periodicals LLC. Basic Protocol 1 : Live imaging in the mouse liver Support Protocol : Monitoring vital signs of the mouse during live liver imaging Basic Protocol 2 : Visualizing blood and bile transport using intravital microscopy