Vancomycin functionalization of gold nanostars for sensitive detection of foodborne pathogens through surface‐enhanced Raman scattering. Issue 12 (9th December 2022)

• Record Type: Journal Article
• Authors: Dayalan, Sandhiya; Gedda, Gangaraju; Li, Ruei–Nian; Zulfajri, Muhammad; Huang, Genin Gary
• Is Part Of: Journal of the Chinese Chemical Society. Volume 69:Issue 12(2022)
• Page Start: 2049
• Page End: 2060
• Publication Date: 2022-12-09
• Subjects: food–borne pathogens -- gold nanostars -- surface‐enhanced Raman scattering (SERS); Chemistry -- Periodicals; Electronic journals; 540.5
• DOI: 10.1002/jccs.202200421
• Languages: English
• ISSNs: 0009-4536

Abstract

Abstract: In this paper, we report a sensitive surface‐enhanced Raman scattering (SERS) biosensor for the detection of food‐borne pathogens on the basis of recognition of bacterial cells through the specific targeting agent vancomycin. Vancomycin‐functionalized gold nanostars (GNSs–4–MBA–van) were synthesized as SERS tags for specific and sensitive quantification of food pathogens. The Raman reporter molecule 4–mercaptobenzoic acid (4–MBA) was also a linker molecule to attach vancomycin to gold nanostars. The proposed vancomycin‐based SERS technique achieved a detection limit of 5.7 and 8.2 CFU/ml for Staphylococcus aureus and Escherichia coli, respectively. The logarithm of the bacterial concentration ranged from 10 1 to 10 7 CFU/ml and demonstrated a linear relationship with the SERS intensity of the characteristic 4–MBA Raman peak at 1, 081 cm −1 . Principle component analysis was used to discriminate the two pathogens simultaneously by using reproducible and specific intrinsic fingerprints and intracellular components obtained through SERS. Moreover, the spiking tests revealed that GNSs–4–MBA–van is effective as a potential biosensor for detecting pathogens in the fresh meat and food‐processing industries. Abstract : A new surface‐enhanced Raman scattering (SERS) biosensor was developed based on green synthesized Au‐seed mediated gold nanostars. Subsequently, gold nanostars were modified with vancomycin as recognition phase and immobilized with 4‐Mercaptobenzoic acid as Raman reporter as well as linker for vancomycin conjugation. Finally, bacteria were determined by utilizing the bacteria's high affinity with vancomycin and measuring the signal intensity elevation of SERS tags.