Acquisition of genomic elements were pivotal for the success of Escherichia coli ST410. (7th October 2022)

• Record Type: Journal Article
• Authors: Chen, Liang; Peirano, Gisele; Kreiswirth, Barry N; Devinney, Rebekah; Pitout, Johann D D
• Is Part Of: Journal of antimicrobial chemotherapy. Volume 77:Number 12(2022)
• Page Start: 3399
• Page End: 3407
• Publication Date: 2022-10-07
• Subjects: Anti-infective agents -- Periodicals; Chemotherapy -- Periodicals; 615.58
• DOI: 10.1093/jac/dkac329
• Languages: English
• ISSNs: 0305-7453

Abstract

Abstract: Background: Escherichia coli ST410 is an emerging MDR clone linked to bla CTX-M-15 and bla OXA-181 . Limited comprehensive data about the global distribution of ST410 clades and mobile genetic elements associated with different β-lactamases are available. Methods: Short- and long-read WGS were performed on a collection of ST410 producing carbapenemases ( n = 45) obtained from 11 countries. The evolutionary history of global E. coli ST410 was also investigated. Results: OXA-181 and NDM-5 were the most frequent carbapenemases and used different underlying strategies to ensure their successful association with ST410 clades. Our phylogenetic analysis of publicly available ST410 genomes amended the previously published ST410 B subclades: ST410-B1 is identical to B1/ H24, ST410-B2 includes B2/ H24 R and B3/ H24 Rx, while ST410-B3 corresponds to B4/ H24 RxC. Long-read WGS identified the following genomic events that likely shaped the evolution of ST410-B3: (i) gyrA and parC mutations were acquired via homologous recombination events; (ii) chromosomal integration of bla CMY-2 among ST410-B3; (iii) the emergence of ST410-B3 from ST410-B2 was accompanied by the replacement of IncFII plasmids harbouring bla CTX-M-15 (i.e. F36:31:A4:B1 in ST410-B2 with F1:A1:B49 plasmids in ST410-B3); and (iv) the NDM-5 gene was integrated within F1:A1:B49 plasmids over time. Conclusions: The global ST410 population producing carbapenemases is dominated by the ST410-B2 and B3 subclades with varied geographical distribution that requires ongoing genomic surveillance. We provided an updated timeline of pivotal genomic events that have shaped the success of the ST410-B3 subclade.