A comprehensive GlobalFiler™ autosomal STR reference dataset for Southern Africa. (December 2022)

• Record Type: Journal Article
• Authors: Kasu, Mohaimin; Lesaoana, Mpasi; D'Amato, Maria Eugenia
• Is Part Of: Forensic science international. Volume 8(2022)
• Page Start: 234
• Page End: 236
• Publication Date: 2022-12
• Subjects: GlobalFiler™ STR panel -- Southern Africa -- Autosomal DNA profiles -- Novel variants; Forensic genetics -- Periodicals; Forensic Genetics -- Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.fsigss.2022.10.046
• Languages: English
• ISSNs: 1875-1768

Abstract

Abstract: In this study a total of n = 832 autosomal DNA profiles from Southern Africa are analysed using the GlobalFiler™ STR panel. The dataset includes South Africa (SA) profiles (n = 541) produced by Ristow et al. 2016 and includes newly generated data for SA Sepedi (n = 96) and Lesotho populations (n = 195). For the newly generated (n = 291) genotypes, we report a large degree of rare and novel variation. This included (n = 7) off-ladder allele variants and (n = 7) TPOX tri-alleles. We report forensic summary statistics and genetic diversity parameters. Expected heterozygosity and observed heterozygosity ranged between (0.7– 0.9) with SE33 as the most polymorphic and TH01 the least. For SA and Lesotho genotypes the combined match probability was (1.13 ×10 -24 and 6.035 ×10 -24 ) and the combined paternity index (1.4 ×10 9 and 2.44 ×10 8 ) respectively. The power of exclusion (0.9999) was similar for each dataset and no significant departures from Hardy-Weinberg equilibrium (HWE) were observed after Bonferroni correction. Population comparisons were performed by MDS and neighbour-joining and population structure inferred by STRUCTURE and DAPC unsupervised clustering.