Isolation and identification of a newly isolated lipase-producing bacteria (Acinetobacter baumannii RMUTT3S8-2) from oily wastewater treatment pond in a poultry processing factory and its optimum lipase production. (December 2022)

• Record Type: Journal Article
• Authors: Bunmadee, Sunanta; Teeka, Jantima; Lomthong, Thanasak; Kaewpa, Dolnapa; Areesirisuk, Prapatsorn; Areesirisuk, Atsadawut
• Is Part Of: Bioresource technology reports. Volume 20(2022)
• Publication Date: 2022-12
• Subjects: Acinetobacter baumannii -- Lipase -- Enzyme stability -- Oily wastewater -- Poultry processing factory; Biomass energy -- Periodicals; Biotransformation (Metabolism) -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Waste products as fuel -- Periodicals; Waste products as fuel; Organic wastes; Factory and trade waste; Biotransformation (Metabolism); Biomass energy; Agricultural wastes; Periodicals; Electronic journals; 662.88
• DOI: 10.1016/j.biteb.2022.101267
• Languages: English
• ISSNs: 2589-014X

Abstract

Abstract: This research aimed to optimize the cultural conditions of a newly isolated RMUTT3S8-2 from oily wastewater treatment pond in a poultry processing factory to enhance its lipase production. The isolated RMUTT3S8-2 was molecularly identified as Acinetobacter baumannii . Lipase production was optimized using Plackett-Burman Design and Box-Behnken Design. The important variables were investigated and chosen as peptone, 24.49 g/L; yeast extract, 33.82 g/L and NaCl, 6.21 g/L. In optimized conditions, the lipase activity of A . baumannii RMUTT3S8-2 was 216.23 ± 3.69 U/mL, more than unoptimized condition by 2.2 times. Lipase stability was above 95 % in the pH range of 5.0 to 9.0 at 30 °C. In addition, A . baumannii RMUTT3S8-2 could digest the oil in poultry processing wastewater and exhibited the degree of oil hydrolysis of 41.94 ± 4.98 % at 12 days. This newly isolated A . baumannii RMUTT3S8-2 showed promise as a lipase producer, especially for oily wastewater treatment. Graphical abstract: Fig S1. Flow chart of the experimental method in this research. Unlabelled Image Highlights: A. baumannii RMUTT3S8-2 was newly isolated from an oily wastewater treatment pond. Lipase production increased 2.2 times under optimal conditions. Stability of produced lipase was higher than 95 % in pH 5.0 to 9.0. A. baumannii RMUTT3S8-2 digested oil in poultry processing wastewater.