Pre-Conceptional Exposure to Glyphosate Affects the Maternal Hepatic and Ovarian Proteome. (29th September 2022)

• Record Type: Journal Article
• Authors: Novbatova, Gulnara; Timme, Kelsey; Severin, Andrew; Sayadi, Maryam; Keating, Aileen F
• Is Part Of: Toxicological sciences. Volume 190:Number 2(2022)
• Page Start: 204
• Page End: 214
• Publication Date: 2022-09-29
• Subjects: glyphosate -- ovary -- proteome -- pregnancy; Toxicology -- Periodicals; Toxicology -- Periodicals; Toxicology; Periodicals; 615.9
• DOI: 10.1093/toxsci/kfac098
• Languages: English
• ISSNs: 1096-6080

Abstract

Abstract: Exposure to glyphosate (GLY), a commonly used herbicide, is supported by urinary detection and associated with shortened gestation in women. This study tested the hypothesis that chronic low-dose pre-conceptional GLY exposure would affect maternal ovarian function mid- and post-gestation. Mice (C57BL/6; n = 40) were exposed per os to saline vehicle control (CT; n = 20) or GLY (2 mg/kg; n = 20) daily for 10 weeks starting at 7 weeks of age. Post-exposure, females were impregnated and euthanized at gestation day 14 (GD14) or post-weaning (PW). Pregnancy success was reduced from 75% to 55% by GLY exposure. No treatment effect ( p > .05) on body weight, maternal serum 17β-estradiol, or litter size was noted. Ovarian weight was unaffected or reduced ( p < .05) by GLY in GD14 and PW dams, respectively. Exposure to GLY decreased ( p < .05) PW ovarian secondary follicle number with no other follicle composition impacts. Protein abundance analysis by LC-MS/MS identified that GLY altered ( p < .05) 26 ovarian and 41 hepatic proteins in GD14 dams and 39 hepatic proteins in PW dams. In GD14 dams, GLY increased ovarian protein abundance of SEC16A ( p < .05; 29-fold) and hepatic RPS27L and GM4952 ( p < .05; ∼4-fold). In both GD14 and PW dams, GLY exposure increased ( p < .05) hepatic RPS4 and decreased ( p < .05) ECHDC3. Pathway analysis using DAVID identified 10 GLY hepatic pathway targets with FDR ≤ 0.07 in GD14 dams.