In vitro Applications of the Terpene Mini‐Path 2.0. (17th November 2022)

• Record Type: Journal Article
• Authors: Couillaud, Julie; Amouric, Agnès; Courvoisier‐Dezord, Elise; Leydet, Létitia; Schweitzer, Nicolas; Rosso, Marie‐Noëlle; Hage, Hayat; Loussouarn‐Yvon, Margot; Vincentelli, Renaud; Petit, Jean‐Louis; de Berardinis, Véronique; Attolini, Mireille; Maresca, Marc; Duquesne, Katia; Iacazio, Gilles
• Is Part Of: Chembiochem. Volume 23:Number 24(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-11-17
• Subjects: cyclobutyl terpenoid derivatives -- farnesyl diphosphate -- terpene mini-path -- tryprostatin B -- (+)-δ-cadinol; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202200595
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: In 2019 four groups reported independently the development of a simplified enzymatic access to the diphosphates (IPP and DMAPP) of isopentenol and dimethylallyl alcohol (IOH and DMAOH). The former are the two universal precursors of all terpenes. We report here on an improved version of what we call the terpene mini‐path as well as its use in enzymatic cascades in combination with various transferases. The goal of this study is to demonstrate the in vitro utility of the TMP in, i) synthesizing various natural terpenes, ii) revealing the product selectivity of an unknown terpene synthase, or iii) generating unnatural cyclobutylated terpenes. Abstract : A generic platform "TMP 2.0" is described for in vitro natural and non‐natural terpenoid enzymatic synthesis.